Temperature-dependent staining reaction of chromatin by Alcian blue.

نویسندگان

  • J C Stockert
  • A Juarranz
چکیده

After treatment with Alcian blue at high temperatures, nuclei from chicken blood smears showed an intense staining reaction, which proved to be dependent on the DNA content of chromatin. The possibility that interactions between the phthalocyanine chromophore and unpaired bases accounts for this temperature-dependent staining of chromatin is briefly discussed.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

A new staining method using a hematoxylin, alcian blue and periodic acid-Schiff reaction for demonstrating mucins in gastric glands.

Mucous neck cells in the rat stomach which contain neutral mucin were stained with a modified Mayer's hemalum. When alcian blue staining and periodic acid-Schiff (PAS) reaction preceded hematoxylin staining, mucin-containing fundic gland cells could be distinguished in the same tissue sections: surface mucous cells were positive for PAS reaction and/or alcian blue staining, neutral mucin-contai...

متن کامل

Effects of Oxidation upon Alcian Blue Staining of Acid Mucopolysaccharides.

Since Steedman ( 1950) has introduced it into histochemistry, alcian blue has been advocated to reveal a staining selectivity which depends upon the presence of acidic groups in mucopolysaccharides (Mowry, 1956; Pearse, 1960). As a plausible ground of this concept the fact has been presented that the alcian blue staining of polysaccharides can be increased in intensity, when acidic groups are i...

متن کامل

Alcian dyes in calcium chloride: a routine selective method to demonstrate mucins.

One of the simplest procedures to demonstrate mucinous substances (acid mucopolysaccharides) in routine histological preparations involves the use of Alcian dyes. Methods involving these dyes have largely. replaced the older, more empirical mucicarmine technique arid are faster and less complicated than the colloidal iron method of Hale,1 which suffers from the possibility of false-positive sta...

متن کامل

Two Separated Protocols with the Most Important Comments for Skeletal Staining in Embryonic and Adulthood Period in Laboratory Animals

Introduction: Skeletal staining is an important part of anatomical studies which can evaluate macroscopic disorders in bones and cartilages development. The aim of the present article is to illustrate two distinct protocols by all details in order to stain skeletal system of the lab animals in all ages of their lives so it becomes easy for future studies in this field. Methods: In this pa...

متن کامل

Differentiation and Proliferation of Embryonic Mast Cells of the Rat

Histochemical reactions and radioautography were used to investigate the sequence of mast cell development in rat embryos. Mast cells arise ubiquitously in and are confined to the loose connective tissue in the embryo. The alcian blue-safranin reaction distinguishes between weakly sulfated and strongly sulfated mucopolysaccharides by a shift from alcian blue to safranin staining. Based on this ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Zeitschrift fur Naturforschung. Section C, Biosciences

دوره 35 11-12  شماره 

صفحات  -

تاریخ انتشار 1980